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Beitragstitel Analysis of tear proteome in Graft versus Host Disease patients of varying disease severity
Autor:innen
  1. Olivia O'Leary Universitätsspital Basel Präsentierende:r
  2. Nadine Gerber-Hollbach Universitätsspital Basel
  3. Kim Plattner Universitätsspital Basel
  4. Alexander Schmidt Universität Basel
  5. Suzette Moes Universität Basel
  6. Paul Jenoe Universität Basel
  7. Christoph Ullmer F. Hoffmann-La Roche Ltd.
  8. Beatrice Drexler Universitätsspital Basel
  9. Jörg Halter Universitätsspital Basel
  10. David Goldblum Pallas Kliniken
Präsentationsform Free Paper
Themengebiete
  • External Disease / Cornea
  • Orbit / Lids / Lacrimal System
Abstract-Text Purpose: Ocular graft versus host disease (oGVHD) is a common complication that occurs in approximately 50% of patients following allogenic hematopoietic cell transplantation (AHCT) and is characterized by a severe dry eye phenotype. To date, no oGVHD biomarkers have been validated for clinical use.
Methods: Forty nine patients with and without chronic oGVHD after AHCT were recruited. Patients were stratified according to the NIH classification guidelines for severity of oGVHD as follows: NIH 0 (none; n = 14), NIH 1 (mild; n = 9), NIH 2 (moderate; n = 16), NIH 3 (severe; n = 10). The proteomic profile of tears collected onto Schirmer strips was analysed using liquid chromatography tandem mass spectrometry. Expression data of the top 20 differentially expressed proteins in oGVHD patients identified in a previous study were compared across disease severity groups. Statistical analysis was performed by Kruskal Wallis test and Dunn’s post hoc analysis.
Result: Significant reduction of lacrimal gland proteins LTF, LACRT, LYZ, PIP, SCGB2A1 and SCGB1D1 was detected in patients with NIH scores of 2 and 3, but not those with NIH score 1. Similarly, progressive downregulation of expression of extracellular proteases CST1 and CST4 was observed in NIH groups 2 and 3. Upregulation of actin-binding proteins MYH14, TMSB4X, ARPC4 and CAPN1 was detected in moderate and severe oGVHD cases. Significant upregulation of cornification envelope protein PPL and nuclear envelope protein LMNA was observed in GVHD patients of all severity scores. No significant changes were identified between the severity groups NIH 1, 2 and 3.
Conclusion: Significant differential protein expression is seen in moderate and severe cases of oGVHD. In the present study, no clear early biomarkers for oGVHD pathology were identified.